HTRF is a proprietary time-resolved fluoresce nce technology that overcomesThe SpectraMax i3x reader features a cooled photomultiplier tube (PMT) for low background noise in luminescence. Nuclear Receptor. Bound crystal violet was dissolved with 10% acetic acid and the optical density (OD) at 570 nm was determined using a Spectra Max i3X spectrophotometer (Molecular Devices). By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Microplate Stacker. 303 unable to cal dark current. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. detection cartridge LUM96: 59. D 1 1. eu@moldev. Italic and boldface type are used for emphasis. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Expand your lab’s capabilities to include flash applications such as dual luciferase and. More Product Information. Features. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. SpectraMax M5. Unlock your i3/i3x instrument after shipment. Note: Additional readers with similar performance are listed. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. , 2014; Xiong et al. SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. Prime function 20 amol. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. SpectraMax i3x microplate reader and SoftMax Pro Software protocol. GFP expression imaged on the cytometer. Anne T. Promega. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The fluorescence spectra were acquired using a microplate reader (Spectra Max i3x; Molecular Devices, USA). SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the datathe SpectraMax i3x Multi-Mode Microplate Reader with SpectraMax Injector Cartridge Introduction Reporter gene assays are used to study the expression of eukaryotic genes. It allows you to explore cellular . Patented user-exchangeable cartridge design expands the system's detection capability making it. Thetypeof luciferase(Renilla. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Home > Search Results > Danaher Inc > spectramax i3x. 00800 665 32860. china@moldev. After incubation at 37 °C and 5% CO 2 for 20 min, fluorescence images were taken using a Spectra Max i3x with MiniMax 300 Imaging Cytometer (Molecular Devices, San Jose, CA, USA) with the excitation and emission setup provided with SoftMax Pro 7. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 2 only) SpectraMax M-series Microplate readers. With optimized reagents, option to operate the readers. . With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyWe would like to show you a description here but the site won’t allow us. TUNE Tunable Wavelength Detection Cartridge. 314 low lamp level warning. 발광 (Luminescence) 리더기. Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. applications, including dual luciferase and ATP assays. 303 unable to cal dark current. Both Microplate Optimization and Read Height Adjustment were. Microplate readers, multi-mode, SpectraMax® i3x. . Find regional support. If you do not have an iLab account you must first make one: SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. 2. . Bioz Stars score: 98/100, based on 1 PubMed citations. An MPO assay has been performed to quantify the inflammation reduced by the GAR-PLGA-ES100 NPs in the inflamed cells . 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. The corrected absorbance is within 5% of the value. 6% for the on-board optics (monochromators). Videos & Webinars SpectraMax i3x - Detection Module ChangeSpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. 305 reference level saturation. Controls containing all reaction components with either DI . For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. Register to download your free eBook today. Other services inlcude but are not limited to anti-drug activity (ADA), PK/PD assays as well as antibody screenings. The device can be upgraded to include capabilities for Western blotting, imaging and injection. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. The SpectraMax i3x from Molecular Devices is a multi-mode plate reader that can measure spectral-based absorbence and luminescence. Modularity does provide some measure of forward vision into upcoming or future experimental needs. HTRF IP standard curve. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Manufacturers try to design their instruments so they can be. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Multimode Reader Comparison Chart. SpectraMax i3x Multi-Mode Detection Platform. SpectraMax i3Instrumenttovisuallyinspectyoursampleandtoruncell-basedassaysat cellularorwhole-cellresolution. About the reader setup recommendations The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The chemical reduction of resazurin was evaluated incubating each well of the microtiter plate with 10 μL (10% of final well volume) of 0. The treatments were. Flow cytometry was used to measure ROS concentrations, based on fluorescence intensity (FL-1, 530 nm) of 10,000 cells. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. 13. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic G q -coupled receptor. o For example: Ex: 485/Em 525/Cutoff 515 4. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. 3. ValitaTiter Plus settingsThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. pathways and protein activation and expression in one system. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. 蛍光 発光 ELISA. The turbidity. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Promega. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. A: percent GFP-positive cells calculated using the classification feature in the software. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. 3: The cuvette chamber. 01 0. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Eliminate time-dependent substrate addition steps. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthThe SpectraMax i3x reader grows with you as your research areas expand. Data Analysis. Examples: fiPress carefully to engage,fl fiDo not press down. The chamber. Capture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. Molecular Devices are known for both the reliability and the wide uses of their products. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 190 control panel are shown in boxed Helvetica type. Read height should be optimized for . 4. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves. Spectranet customer portal The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The chamber. After incubation at 37 °C and 5% CO 2 for 20 min, fluorescence images were taken using Spectra Max i3x with MiniMax 300 Imaging Cytometer (Molecular Devices, San Jose, CA) using the setup of excitation and emission provided in the SoftMax Pro 7. 蛍光 発光 ELISA. ATP (“flash” luminescence using . The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax i3x reader grows with you as you continue to advance your research, enabling you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. HTRF IP standard curve. 4. Find regional support. 315 can't find zero order. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Microplate reader assays requiring infrared (IR) or red-shifted. 2. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. The plate was shaken and incubated in the dark for 10 minutes. each new lot of plates, to ensure that the center of each well is read. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. SpectraMax iD5:Fluorescence was quantified using a microplate reader (SpectraMax i3x, Molecular Devices, CA, USA) with an excitation and emission wavelength being 365 nm and 450 nm, respectively (Jian et al. The TRF intensities measured are proportional to the level of extracellular acidification in each sample. Key features. 1) Select the correct plate and the wells that you want to measure. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. #0200-7009) The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. SpectraMax i3x, onboard luminescence 121. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Example: [Drawer]. Description 1. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology:. Fluorescence Reader. Increased fluorescence performance. Spectranet customer portalPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. The SpectraMax ® i3x reader provides the. Kastelic,2 and Jian Gao 1 1Department of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. The SpectraMax® i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6-, 384- well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. 3 In this application note we demonstrate a linear dynamic range of 6 decades for recombinant firefly and Renilla luciferases, as well as linear detection of luciferase-transfected. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. , 2011). Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. SpectraMax i3x Multi-Mode Microplate Reader SystemSpectraMax® i3x dari Molecular Devices adalah sistem deteksi berbasis multi-mode yang berevolusi menyesuaik. 3: The cuvette chamber. Molecular Devicesの全商品を見る. 0. techsupport. 99 to $1999. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Item SpectraMax i3x Multi-Mode Microplate Readers; Company Molecular Devices LLC; Catalog Number SpectraMax i3x <Disabled> This product is no longer available on Biocompare. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologySpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. User-upgradeable detection modules allow you to expand the capabilities of your microp. 3) At the bottom of the strip, click on "Show. 7 mm for all shake intensities. applications, including dual luciferase and ATP assays. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable. 10, and 0. As shown in the results above, the DELFIA cell proliferation assay combined with the SpectraMax i3 reader gives researchers a very sensitive test for measuring cell proliferation. The SpectraMax i3x reader provides theThe SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer can be used to image slides using a specialized slide adapter. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Contact Local Team. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. To read HTRF®, the SpectraMax i3x® must be first equipped with the SpectraMax i3x® Cisbio HTRF® cartridge, which enables the simultaneous measurement of both 620nm donor and 520 nm acceptor emissions. Pick the best speed, sensitivity, and price for your needs. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. We would like to show you a description here but the site won’t allow us. ). 2. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. We would like to show you a description here but the site won’t allow us. 5 decades, with sensitive detection of cells. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. The assay window spanned four orders of magnitude. 5577 Web: Email: [email protected] SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. The plate was covered and incubated for one hour at room temperature. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Simplified workflow: Add cellular imaging assays in a small footprint. 0´ 105 dsD NA (p g/ wel ) R F USpectraMax Glo Steady-Luc 工作液都加 入到微孔板后，震荡摇匀并避光放置 10 min。使用 SpectraMax Glo Steady-Luc Reporter 内置的 SpectraMax Glo Steady- Luc Reporter 实验模板设定程序并通过 SpectraMax i3x 读板机检测发光。检测结 束后，模板会根据数据自动生成一条曲 线。SpectraMax MiniMax 300 细胞成像系统 为 SpectraMax i3x 多功能微孔板检测平台而打造！ • • • • 主要特点 系统全面兼容细胞成像 及微孔板检测技术 独特无标记分析技术无需 对细胞染色即可进行细胞 计数和贴壁度的计算 具有多种检测功能可对 细胞活力及毒性进行全. Structured Review. After correcting for negative controls and quench standards, the soil enzyme activities were expressed in units of nmol h −1 g −1 soil (German et al. Image analysis provides accurate data. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. no. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SoftMax Pro Software quickly. The SpectraMax i3 also allows the user to identify antibodies during Elisa Assays. 0´ 104 1. *On the SpectraMax i3x reader only. 0. Prime function 20 amol. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. The SpectraMax® i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6-, 384- well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. Figure 1. SpectraMax i3/i3x readers must be equipped with a suitableWhen we measured the FRET ratio using the Spectramax i3x plate reader, we found that FRET ratios were higher in DMSO-treated cells that had not been incubated at room temperature to enhance fluorescence than in room temperature-incubated cells (Fig. Category 2 and 3 microplate readers use gradient heating as well. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 1. Introduction. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5e Fluorescence was quantified using a microplate reader (SpectraMax i3x, Molecular Devices, CA, USA) with an excitation and emission wavelength being 365 nm and 450 nm, respectively (Jian et al. Here, we demonstrate the benefits of using the SpectraMax ® i3x Multi-Mode Microplate Reader with the SpectraMax ® MiniMax™ 300 Imaging Cytometer in combination with MetaMorph ® Microscopy Automation and Image Analysis Software for performing automated neurite outgrowth imaging and analysis. Prepare your i3/i3x instrument for shipment. 1 mg/mL density of resazurin, protected of the light, incubated for 1 h at room temperature. This instrument also provides the option of modular upgrades for Western Blotting,. 1. 흡광 (Absorbance) 리더기. Louis, MO) and used as received without further purification. flValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. Recommendations for SpectraMax i3/i3x plate readers The Molecular Devices SpectraMax i3/i3x allows use of the pH-Xtra Glycolysis Assay with time-resolved fluorescence (TRF) detection. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. 306 plate air cal. Microplate Readers SpectraMax. The dual auto injector module. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. Multimode Readers Comparison Chart. 2C). ; Page 2 Molecular Devices equipment, software, reagents, and consumables. SpectraDrop Abs Protein Quant (i3-i3x)Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Spectranet customer portalSpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Spectranet customer portalWatch this video to get an introduction to the Injector-Ready SpectraMax® i3x Multi-Mode Microplate Reader System. Red and green fluorescence was then measured (emission at 590 and 530 nm, respectively, with excitation at 490 nm) using a fluorescence microplate reader (Spectra Max i3x, Molecular Devices Inc. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Introduction. Find regional support. Turbidity measurements were performed with a Spectra Max® i3x microplate reader to study the effect of oxidation on the kinetics of sFN formation. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. 316 grating motor driver faulty. Contact Us Phone: +1. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. 5. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Molecular Devices, LLC Reading Modes: Endpoint and Kinetic; Optical System: Spectral Fusion Illumination. Danaher Inc spectramax i3x. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. ; The top 3 importers of Spectramax are India with 365. Italic and boldface type are used for emphasis. 312 gain calibration failed. Get a front row view of your cells. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. It features a modular design that allows users to add or change detection cartridges according to their needs. 4 / 5. Examples: fiPress carefully to engage,fl fiDo not press down. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . The fluorescence spectra was obtained on a Spectra Max i3x Multi-Mode Detection Platform. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. 304 signal level saturation. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. i3. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. Synthesis of NORP Synthesis of compound 1. , 2011). 19 Switch on the SpectraMax i3x plate reader and launch SoftMax Pro software. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 12 mg/mL) of polysaccharides were prepared after addition of glucosum anhydricum for calibration. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. 315 can't find zero order. The calculated LOQ values were 0. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. Get a front row view of your cells. Replicate values were averaged and imported into Design-Expert 8. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. To detect physical stability, Pdots were treated with water and incubated with plants. Then, the cell area was calculated for each. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. 価格、見積もり、お近くの販売店を. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes This assay workflow is easily performed using the SpectraMax i3x Multi-Mode Microplate Reader with SpectraMax Injector Cartridge, which is fully DL Ready validated. 2 in. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely low light. Settings not required by a reader are indicated by ‘---’. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. It allows the user to determine the total concentration of protein in a solution. SpectraMax ® iD3 and iD5 Multi-Mode . Dependingontheapplication,theinstrumentcanread6,12,24,48,96,and384-well microplates. SpectraMax i3x. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. Description 1. The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. 314 low lamp level warning. StainFree Cell Detection Algorithm. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Besides, it’s possible to examine each page of the guide singly by using the scroll bar. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. SpectraMax i3x Multi-Mode Detection Platform. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. However, no piece of equipment is immune to breaking down. Multimode Reader Comparison Chart. High resolution X-ray photoelectron spectroscopy (XPS) of cCQD was obtained on a K-Alpha+ (Thermo Fisher Scientific, Massachusetts, USA) spectrometer. We would like to show you a description here but the site won’t allow us. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. Amplitude : 1. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. at 540 nm using a Spectra Max i3x microplate reader (Molecular devices, San Jose, CA). Multimode Readers Comparison Chart. 4 μM), prepared as described in section 2. The information below applies to a standard 96 well plate, set to a linear shake mode. 8 Antibodies and. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel.